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Thawed spermatozoa were separated using a Percoll density gradient (45%/90%) and capacitated for 15 min at 38.5 C and 5% CO 2 in fertilization medium

El Kasmi KC, Stenmark KR

( Epub 20220304 )

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It has been shown that multiple apoptosis-regulating proteins are substrates for lysosome-derived cathepsins, including Bid, Bcl-2, Bcl-XL, and XIAP [62]