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Cryo-EM data acquisition The concentrated sample (3.5 L) was applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3, 300 mesh), and subsequently vitrified using a Vitrobot Mark IV (ThermoFisher Scientific) set at 100% humidity and 4C

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Compared to the placebo group, the continuous treatment group had fewer days of acute exacerbation, while no difference was found between the intermittent group and the placebo group in terms of acute exacerbations

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