OCR was recorded three times under basal conditions and three times after the addition of Mito Stress compounds (Mito Stress Test Kit, Agilent, Santa Clara, California, USA): oligomycin (oligo, 1 M, adenosine triphosphate (ATP)-synthase inhibitor), fluorocarbonyl cyanide phenylhydrazone (FCCP, 0.5 M, decoupler), and rotenone/antimycin A (rot/AA, 0.5 M, complex 3/1 inhibitor), as shown in Figure 1
We follow proper clinical protocols not just a quick jab and out the door
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Decant the supernatant and resuspend the washed pellet in 12 ml U-buffer (resuspend in 20 l U-buffer per ml of original culture)
two 50 mg vials cover the cycle with headroom