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For ER36 expression, 1 10 6 cultured breast cancer cells or cells from xenograft tumors were incubated with 10 L of anti-ER36 antibody at 4 C for 40 min, followed by incubation with 0.5 L goat anti-mouse Alexa Fluor 647 antibody (Sparks, MD) at 4 C for 30 min

doi:10.1080/01443615.2016.1264375 Salehpour S, Nazari L, Hoseini S, Moghaddam PB, Gachkar L

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Hanna, M., Jaqua, E., Nguyen, V., & Clay, J

Preclinical evidence suggests that increasing protein intake may offset circulating amino-acids reduction, however this may also blunt the increase in whole-body energy expenditure [63]