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Mpemba, and I am going to tell you about my discovery, which was due to misusing a refrigerator. Thus begins a 1969 paper in the journal Physics Education in which Mpemba described an incident at Magamba Secondary School in Tanzania when he and his classmates were making ice cream

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How should reconstitution solutions be stored

Sterile water works best when: The entire reconstituted peptide will be used in a single session Maximum purity without any additives is required for the protocol Cell culture experiments demand preservative-free conditions Budget constraints favor the more economical single-use option Sensitive assays might show interference from benzyl alcohol Research Application Considerations Cell culture applications present unique constraints on reconstitution solution selection

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