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For biotinylation, 4.5 mg mL 1 of TGP Avi was incubated with 0.13 mg mL 1 of His-tagged BirA, 0.72 mg mL 1 His-tagged 3C protease, 5 mM ATP, 10 mM magnesium acetate, 0.23 mM biotin in 500 L volume and incubated at 4 C for 11 h