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To selectively activate endogenous GLP-1-producing NTS neurons that project to the VTA, the retrogradely infecting virus CAV2 expressing Cre recombinase (CAV2-Cre) was infused into the VTA and a Cre-dependent virus expressing a neural activating DREADD (AAV-DIO-hM3D(Gq)-mCherry) was infused into the NTS (Fig 2A & 2B)

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