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IL-1, interleukin-1, IFN, interferon

Cells were incubated with antibodies at a 1:100 dilution, or as specified otherwise, and were stained at RT for 15 min, washed, and analyzed using CytoFLEX and Kaluza software version 2.1 (Beckman Coulter, Milan, Italy) or using the Sony ID7000 Spectral Cell Analyzer equipped with 5 lasers (355 nm, 405 nm, 488 nm, 561 nm, 637 nm)

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The fundamental formula is straightforward: (Desired Dose in mcg / Concentration in mcg/mL) = Volume to Administer in mL Lets walk through a practical example

Some people find that applying gentle pressure with a cotton ball immediately after needle removal also helps

For a 2mg dose: 2 / 50 x 100 = 4 units