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Intracellular ATP content The intracellular ATP content was measured using the ATPlite Luminescence ATP Detection Assay System (Perkin Elmer, Waltham, MA, USA), according to the protocol provided by the manufacturer, and the luminescence was measured using a Tecan Genios microplate reader (Thermo Fisher)

(She left Community Health Network in November 2025 to spend more time with her family, according to a spokesperson.) All three doctors declined to be interviewed or did not respond to our requests for an interview

Thus, cystine limitation suppressed mTORC1 and activated the ISR, the two major amino acid sensing pathways in HepG2 cells

*Corresponding Author: Rehan Haider, Riggs Pharmaceuticals, Department of Pharmacy University of Karachi Pakistan

doi: 10.1007/s40618-015-0266-y 187 TauberMCoupayeMDieneGMolinasCValetteMBeauloyeV