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29 (Holst -Biochem J 1980 - 1982, Bataille ' -FEBS Lett 1982, -Thim -Moody -Peptides 1981)
Reacting at room temperature for 3H, adding 10mL of glacial methyl tert-ether, precipitating a white solid, centrifuging, adding methyl tert-ether into the product, washing for 3 times, drying, and purifying by reversed-phase HPLC to obtain 90mg of semaglutide with the purity of 98.54%, wherein the liquid phase chromatogram is shown in figure 10, the mass chromatogram is shown in figure 11, and the molecular weight of the semaglutide is MS: M/z =4113.16 (M + H) + ) Example 10 Synthesis of semaglutide: 100mg of compound 4 obtained in example 6 and having a purity of about 76.13% was added to an iced freshly prepared 1mL lysate (trifluoroacetic acid: 1,2-ethanedithiol: triisopropylsilane = 95
Park B, Bakbak E, Teoh H, Krishnaraj A, Dennis F, Quan A, et al
It is found in every cell of the body and serves as the first line of defense, with particularly high le
