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Fragments were PCR amplifed and gel extracted with a Zymo gel extraction kit before being assembled into pMXS-puro linearized with BamHI and NotI with a 4-component Gibson reaction

These can include nausea, vomiting, diarrhea, constipation, abdominal discomfort, and reduced appetite

10.1016/j.freeradbiomed.2022.01.012 4 CobineP

therefore, it may help preserve lean mass

In general, glycolytic metabolites upstream of GAPDH were enriched upon high dose VitC treatment, while those downstream were depleted, in line with an inhibition of GAPDH by VitC, ultimately leading to the disruption of glycolysis and TCA cycle also observed in several proteomics studies (Table 4, Fig

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