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High prevalence of exocrine pancreatic insufficiency in diabetes mellitus

[33] Ferreira, B., Mendes, F., Osorio, N., Caseiro, A., Gabriel, A

The mean duration of diabetes was 10.3 years

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Cells were washed three times with TBS-T and incubated with secondary antibodies combining donkey anti-mouse AF555 (Thermo Fisher Scientific) and donkey anti-chicken AF647 (Thermo Fisher Scientific) diluted in TBS-T with 1% NDS for 2 hours at room temperature on an orbital shaker