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Some researchers have used subcutaneous injection of scopolamine combined with a dry environment to establish DED mouse models, and immortalized human HCEC-2 cells were cultured under hypertonic conditions to establish hypertonic cell injury models and measure the levels of intracellular Fe 2+ , cellular ROS, and lipid peroxidation

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To disrupt OM for visualization of nozzles, cells swarming on hard agar with or without copper were scraped into CTT media in a 1.5 ml centrifuge tube

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Even individuals just seeking anti-aging benefits have started to incorporate GH into their hormone replacement therapy protocols