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Specifically, researchers found a reduction in GABA levels in the parieto-occipital cortex and the temporal cortex (regions in the brain)

Yes, it is standard best practice in any research protocol involving injections to rotate sites

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In addition, there is decreased glucose uptake in numerous brain regions and spinal cord of animal models and patients with ALS (Dalakas et al., 1987

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Furthermore, as OLGc are the predominant iron-containing cells in the brain and highly susceptible to ferroptosis, their demise may increase local iron, predisposing to FIN (Nobuta et al., 2019