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After washing two times in IC permeabilization buffer (cat#PB001C, BD Biosciences Pharmingen, CA, US), the cells were centrifuged at 1500 g for 4 min and the supernatant aspirated

These compounds are large peptide molecules, meaning they are broken down by stomach acid and intestinal enzymes rather than absorbed intact into the bloodstream

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Retatrutides side effects are primarily GI-related and manageable for most users