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To validate this hypothesis, we performed in vivo experiments using an allograft mouse model of EMs

Nausea, constipation, and reduced appetite occur in 30-50% of GLP-1 users and typically subside within 4-6 weeks

They also ran flow cytometry (Annexin V/PI staining) and western blot analysis to measure specific survival and death markers: Bcl-2, Bax, caspase-3, and PARP

Following LPS stimulation, RIPK1 and RIPK3 assemble into a kinase complex that mediates phosphorylation-dependent activation of MLKL [82]

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