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We first examined the concentration-dependent effect of PFOS on mitochondrial bioenergetics and discovered that an increase in the PFOS concentration from 100 to 200 M significantly decreased mitochondrial bioenergetics, as evidenced by the decreased ATP production, maximal respiration and spare respiratory capacity, and increased proton leakage (Fig

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Then, the SYBR Green PCR Master Mix (#4309155, Applied Biosystems, Foster City, CA, USA) was utilized to determine the mRNA expression of the target genes

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Gently spread the desired amount from center of the face outward

doi: 10.1016/j.chembiol.2018.06.009 31 LuiJ