Results The BGCs of E-64 and E-64 analogs Directly searching for the biosynthetic gene clusters (BGCs) of E-64 using ATP-grasp enzymes and ABSs is challenging because of the lack of characterized examples in fungi
they share a topology of an intracellular N- and C-terminal loop, and extracellular loops EC1 and EC2 with species-specific phylogenetic differences stimulating differential protection (5660)
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After blocking with 5% skimmed milk at room temperature for 1 hour, the membrane was incubated overnight at 4 C with the following primary antibodies (1:1000 dilution unless otherwise indicated): anti-transferrin receptor (TFRC