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Keep it Dark & Dry: Store the vial in its original box or a dark container to protect it from light, which can also contribute to degradation

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Valkonen and Kuusi used dichlorofluorescein-diacetate as a fluorescent oxidizable substrate (1997)

Journal of radiation research

Specifically, 120 mg of frozen floret powder was resuspended with 1.5 mL of pre-chilled 80% ( v / v ) acetone, vortexed for 1 min, and then clarified by centrifugation at 5,000 g for 5 min

initial results have been reported at an earlier Pernicious Anaemia Society meeting, and these presentations can be found online on the PAS website and / or YouTube