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Neurotherapeutics 18, 252264

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Finally, 100 l of stop solution was added into each well and the absorbance at 450 nm was measured

Furthermore, research published in the journal Obesity Science & Practice highlighted the benefits of semaglutide for long-term weight maintenance, demonstrating sustained reductions in body weight and improvements in obesity-related comorbidities over time

Immunofluorescence staining was performed to assess macrophage polarization, with CD86 serving as a marker for pro-inflammatory M1 macrophages and CD206 for anti-inflammatory M2 macrophages