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Sitting drop crystallization was performed by first pipetting 70 L of precipitant solution into each well as reservoir, followed by depositing 150 nL of the precipitant solution on top of 150 nL of protein with a Crystal Gryphon LCP robot (Art Robbins Instruments)

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Nuclear-to-Cytoplasmic (N/C) Ratio Quantification The N/C ratio of NRF2 was calculated by dividing the mean fluorescence intensity (MFI) of NRF2 in nuclei (DAPI-defined regions) by that in cytoplasm (-tubulin-outlined areas excluding nuclei), using ImageJ Software (Version 1.53c)

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