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Biotin was added to a final concentration of 50 M at the indicated timepoint, and cells were harvested 24 h after doxycycline induction

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VPA-exposed animals showed significantly fewer vertical explorations compared to controls ( P 0.05 )

After incubation of the supernatants with Escherichia coli NO 3 reductase (37, 30 min), 1 ml of Griess reagent (0.5% naphthylenediamine dihydrochloride, 5% sulfonilamide, 25% H 3 PO 4 ) was added

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