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The construct was expressed in Spodoptera frugiperda ( Sf 9) insect cells (Invitrogen) with Bac-to-Bac Baculovirus system (Invitrogen) and cells were infected at a density of 2 10 6 cells per mL and collected 48 h after infection

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Without these characteristics, they cant make melanin like they normally would

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Gmez-ManzoSMarcial-QuinoJVanoye-CarloASerrano-PosadaHGonzlez-ValdezAMartnez-RosasVet alFunctional and biochemical characterization of three recombinant human glucose-6-phosphate dehydrogenase mutants: Zacatecas, Vanua-Lava and Viangchan

I went ahead and tried subcutaneous this morning