Spontaneous activity tests A mouse was placed in a clear glass cylinder and recorded with a video camera for 3 min in a quiet area, to prevent distractions and accidentally causing freezing behaviour
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Notably, 13 genes and their corresponding proteins were upregulated in group H-IMF, including LCN2 (Lipocalin 2), MPO (Myeloperoxidase), CATHL4 (Cathelicidin 4), ACAN (Aggrecan), TTN (Titin), CATHL3 (Cathelicidin 3), CHAD (Chondroadherin), PGLYRP1 (Peptidoglycan Recognition Protein 1), AZU1 (Azurocidin 1), COL2A1 (Collagen Type II Alpha 1 Chain), PRG3 (Proteoglycan 3), and NGP (Neutrophilic Granule Protein)
23 Studies in insulin-resistant and insulin-deficient animal models, as well as humans, continue to highlight the adverse effects of obesity and T2D on bone integrity
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