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Hansen et al [17] used Hanks Balanced Salt Solution (HBSS) containing 5% FCS and 2 mM EDTA for both, while Cervantes-Barragan et al [15] replaced the HBSS with PBS and Luckashenak et al [16] used PBS for suspending the cells and HBSS for diluting the OptiPrep (both containing 0.2% BSA and 2 mM EDTA

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doi: 10.1016/0891-5849(92)90117-y

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After 24 h of reaction, the immobilization carriers were washed three times with 2 mL of superdehydrated acetonitrile, and the superdehydrated acetonitrile was completely volatilized in a centrifugal vacuum concentrator at 30 C