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Immune network dysregulation associated with child neurodevelopmental delay: modulatory role of prenatal alcohol exposure

After 24 h, the medium was discarded, and hepatocytes were cultured in a maintenance medium composed of seeding medium, supplemented by 50 M hydrocortisone hemisuccinate and 2% (vol/vol) DMSO, as previously described ( 41 )

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[PubMed ID: 22549035]

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The 14-day spacing matches the typical 7-to-14-day window controlled microneedling studies use, and it gives the skin barrier full time to recover between sessions