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I watched these medications emerge from early clinical trials, saw firsthand how they transformed glycemic control for patients with type 2 diabetes, and built a working knowledge of this drug class long before it became a household name

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After washing with TBST, membranes were probed with the correspondent secondary antibody for 1 h at room temperature (IMPDH2, LC3, CERT, K-48 polyubiquitin, H2B-Ub and USP-14: anti-rabbit [NA934V], diluted 1:10000 in 5% milk in TBST

Additional information Communicated by Massimo Federici, M.D

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