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J Biol Regul Homeostat Agents (2020) 34(3):83744

The isoelectric point of a solution is reached when the positive and negative charges of amphoteric ions are equal

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PMID 6137189

Some more sensitive compounds don't tolerate the benzyl alcohol preservative well and are better suited to preservative-free sterile water or saline, even though this typically means the reconstituted solution should be used sooner rather than stored for weeks