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Insoluble immune complexes were rinsed once with Co-IP buffer then eluted by 0.1 mM citrate (pH3.0) twice for 5 min, resolved by sodium dodecyl sulfatepolyacrylamide gel, and transferred to nitrocellulose

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In this study, glutathione was provided in supplement form in a dose of 300 milligrams per day for four months

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