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Enzyme activity was calculated using the extinction coefficient of 14.15 mM 1 cm 1 for TNB and the amount of GSH was determined by using a standard curve in which the GSH standard equivalents present (5, 10, 15 and 20 nmol) is plotted against the rate of change of absorbance at 412 nm

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The anti-melanogenic properties of glutathione solution when injected, reduce the production of melanin around the treated area, thus, brightening your skin tone

doi: 10.1186/1999-3110-55-7 52 FujikawaT.IshiharaH.LeachJ