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1 mL samples were collected at predetermined time points (0.5, 1, 2, 4, 8, 12, 24, 48, and 72 h) and then supplemented with the same volume of fresh release medium

(2016) reviewed 54 studies and found that prolotherapy consistently reduced pain and improved function in various ligament injuries

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However, MMA levels can be elevated in those with thyroid and kidney conditions, which limits the tests specificity (9, 10)

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