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SW480 cells treated 5FU for 72 h analysed for mitochondrial mass by NAO staining ( a ) and mtDNA ( b ), n = 3 biological replicates shown with mean s.e.m in both, and mitochondrial protein expression ( c ), blots representative of n = 3 biological replicates

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There is insufficient evidence for the use of B12, beyond correction of deficiency, in cognitive impairment, depression and fatigue