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gRNA-mediated Cas9 double-strand break of genomic DNA between codons tyrosine-972 and leucine-973 should initiate homologous-directed repair using the ssDNA repair oligo, thereby mutating the PAM sequence, exchanging codon leucine-973 to phenylalanine-973, as well as inserting silent point mutations at codons glycine-962 and proline-963 to generate an ApaI restriction site for genotyping
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