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Yes, the copper(II) ion is critical for GHK-Cu's biological activity

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doi:10.1016/j.ejogrb.2021.06.003

While this might partly subcellular compartmentation and the lower reducing power (higher redox potential) of ascorbate compared to glutathione, NADH, and NADPH, it also points to efficient co-operation between the various isoforms of the three reductases

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