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doi: 10.1038/S41385-020-00365-4

The ESI source conditions were set as following: sheath gas flow rate of 50 arbitrary units (Arb), auxiliary gas flow rate of 15 Arb, capillary temperature of 320 C, full MS resolution of 60,000, MS/MS resolution of 15,000, collision energy of 10/30/60 in normalized collision energy (NCE) mode, and spray voltage of 3.8 kV (positive) or 3.4 kV (negative)

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We fused LacAnc100 to the yeast mating protein Aga2p for surface display and used error-prone PCR to create a library of variants with an average of 17 amino acid changes per gene

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