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LCMS metabolomics analysis Sample preparation HCT116s cells (1 10 5 ) treated with or without 5 M 5FU were cultured in DMEM for 24 h and 72 h or supplemented with media containing uniformly labelled 13 C 6 -glucose (25 mM) for 6 h before sampling

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Male study subject, 26 years old, diagnosed with AGA, Norwood IV

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Nature 380:435439 Henry TD, Annex BH, McKendall GR, Azrin MA, Lopez JJ, Giordano FJ, Shah PK, Willerson JT, Benza RL, Berman DS et al (2003) The VIVA trial: vascular endothelial growth factor in ischemia for vascular angiogenesis