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2.9 Cell mito stress test A375 human melanoma cell line (1 10 4 ) was seeded in a 96 well Seahorse XF96 tissue culture Microplate (Agilent Technologies, Santa Clara, CA, United States) 24 h before the assay, then oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) were measured using the Seahorse XFe96 Analyzer, as already described (Lofaro et al., 2020)

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