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To prepare the RNAi-expressing bacteria, single colonies were used to inoculate small cultures (15 mL) before being scaled up to larger cultures in terrific broth (TB) supplemented with 100 g/mL carbenicillin

doi: 10.1074/jbc.M109.090506

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There is no robust evidence that sublingual preparations offer a clinically meaningful advantage over standard oral tablets at equivalent doses