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Sjgren A, Florn CH, Nilsson A
Features of Streptavidin Magnetic Beads: High-performance beads non-aggregating, pre-blocked, iron oxide, superparamagnetic microparticles provide exceptional uniformity for automated HTS and manual applications alike Stable immobilization chemistry streptavidin is immobilized using leach-resistant chemistry High capacity superior quality beads with high binding capacity provide rapid and efficient biomolecule purification from complex samples Low non-specific binding stable, pre-blocked beads provide clean purification products (e.g., antigen eluted in IP with biotinylated antibody) that are compatible with mass spectrometry analysis and other downstream analyses Superior performance nearly three times higher binding capacity than typical beads from other suppliers, allowing the use of smaller amounts per experiment Applications: Immunoprecipitate antigens (using biotinylated antibodies) from a wide variety of sources Co-immunoprecipitate interaction complexes using biotinylated antibodies Capture protein-protein interactions in pull-down assays using biotinylated 'bait' proteins Isolate biotin-labeled DNA-protein complexes from cell or tissue extracts Capture single-stranded biotinylated DNA oligos Isolate biotinylated PCR products These streptavidin magnetic beads are validated and optimized for use with high-throughput magnetic platforms, such as the Thermo Scientific KingFisher 96 and KingFisher Flex Instruments, but the beads also enable premium performance for simple benchtop applications using an appropriate magnetic stand
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