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Peer review involves independent experts evaluating methodology, data analysis, and conclusions before publication

Recommended starting dose: Discontinue other basal insulin and/or GLP-1 agonist before initiating Xultophy Patients nave to basal insulin or a GLP-1 agonist: starting dose is 10 units once daily Patients currently on basal insulin or a GLP-1 agonist: starting dose is 16 units once daily Maintenance: titrate dose up or down by 2 units once or twice weekly, depending on blood sugar goals Missed doses: resume dosing at the next scheduled dose

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Each sample was homogenized using a TissueLyser III (Qiagen) with 5 homogenization beads in 200 l of cold Tris lysis buffer (150 mM NaCl, 20 mM Tris, pH 7.4, 1 mM EDTA, 1 mM EGTA, 1% Triton X) containing a cocktail of protease (cOmplete Protease Inhibitor Cocktail, Roche) and phosphatase inhibitors (Phosphatase Inhibitor Cocktail 2 and 3, Sigma Aldrich), NaF and PMSF in DMSO

Understanding these systems ensures transparency and provides peace of mind throughout the ordering process