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Immunofluorescence microscopy Constructs encoding either wild-type full-length S protein or the clientized Thr1273Glu S mutant, were transfected using jetOPTIMUS transfection reagent (Polyplus, France) into HeLa cells on sterile glass coverslips

Normally, hormones may trigger the release of multiple other chemicals or hormones which causes side effects, but Ipamorelin is more specific in its action and triggers only growth hormone release