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Firstly a few principles

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174,175 Several oncogenic mechanisms, such as the transcription factor HIF-1, PI3K/Akt/mTOR pathway and c-Myc, upregulate genes encoding glucose transporters and glycolytic proteins, including GLUT1, GLUT3, and lactate dehydrogenase (LDH)

Depending on the needs of the study, prepared solutions may be maintained under refrigerated conditions for short-term use or frozen for longer-term laboratory storage, with aliquoting commonly used to help maintain sample integrity during repeated use

Repeat 3 times per year, spaced approximately 4 months apart

Second, the roles of emerging post-translational modifications in mediating this interaction are not fully integrated into current models