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In the case of AF3 (Abramson et al., 2024), the AlphaFoldServer was used with one copy of Chc-NTD and three copies of each peptide for running the predictions

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There is also an emerging interest in brain-type fatty acidbinding proteins, phosphorylated neurofilament H and ubiquitin C-terminal hydrolase, and proteolytic breakdown products of cell proteins such as alpha-II spectrin and the microtubule-binding protein tau (Dash et al., 2010)

Before preparing the cDNAs, all the RNA samples were quantified in nanodrop spectrophotometer (SPECTROstar Nano, BMG LABTECH, Germany) and subjected to DNase (New England Biolabs, USA) treatment following the manufacturers protocol

Subcutaneous bioavailability is estimated at roughly 65% based on phase 1 data, comparable to semaglutide 2.4 mg (Wegovy)