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During I/R injury, gut-derived endotoxins such as lipopolysaccharide (LPS) can translocate from the gut into the systemic circulation, triggering inflammation and immune activation (Suganya and Koo, 2020

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doi:10.1007/s12011-011-9278-4

Acknowledgements This study was approved by the Alfred Hospital Ethics Committee (project number 463/18) and the Australian Institute of Health and Welfare Ethics Committee (EO2018/5/501)

Step 9: Visually Inspect the Solution Hold the vial up to a light source and examine the solution before proceeding: Step 10: Aliquot and Label If you will not use the entire reconstituted volume within one experiment session, aliquot into single-use volumes before storage