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Thus, whilst we could obtain hits from both the NMR and XChem fragment screens, the cross-platform validation was generally limited, which prevented the rational design of larger, more potent analogues

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Following dilutions of antihuman primary antibodies in PBS were applied overnight at 4 C: Nestin (Millipore, MAB5326, 1:200), SOX2 (Abcam, ab97959, 1:200), Doublecortin (Cell Signaling, 4604S, 1:200), MAP2 (Millipore, MAB3418, 1:200), PSA-NCAM (Millipore, MAB5324, 1:200), PSD95 (Abcam, ab18258, 1:200) Tubulin, beta 3 (Millipore, MAB1637, 1:200)

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Naloxone, a blocker of opioid receptors, exacerbated symptoms of OCD, while tramadol, which activates them, relieved OCD symptoms [31]