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TMT liquid chromatography-mass-spectrometry (LC-MS) analysis Samples were analyzed by liquid chromatography-tandem mass spectrometry using an Orbitrap Fusion Tribrid Mass Spectrometer (Thermo Scientific) coupled to an UltiMate 3000 Series Rapid Separation LC system and autosampler (Thermo Scientific Dionex)
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Further, oxidative stress is thought to be responsible for prompting inflammatory processes that can lead to the progression of NAFLD to NASH and cirrhosis (end-stage liver disease) (16)
Moreover, nigella sativa decreases hepatitis viral load, improves response to oxidative stress and improves the clinical condition and glycemic control in hepatitis patients (Box 3)
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