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The standard procedure and reagents from the Q5 Site-Directed Mutagenesis kit (NEB) were used to insert mutations, ligate plasmids and transform them into NEB 5 competent cells by heat shock

Akdis CA (2021) Does the epithelial barrier hypothesis explain the increase in allergy, autoimmunity and other chronic conditions

C.de Azevedo MeloA

The concentration was far below standard GHK-Cu serums (0.02% vs 1 to 2%)

Suzuki, T., Motohashi, H

The oxidative stress is induced by the indirect interactions with the antioxidant defense system, disruption of the electron transport chain, and induction of lipid peroxidation, which are the results of exposure to redox-inactive HMs (Bhattacharyya et al., 2014)