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It is a nutrient strategy developed to support metallothionein and copper-zinc regulation

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Cooke et al., 1991

Improved glycemic control with minimal systemic metformin exposure: effects of metformin delayed-release (metformin DR) targeting the lower bowel over 16 weeks in a randomized trial in subjects with type 2 diabetes

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For the flow cytometry assay, 3 10 4 cells/well/40 L were incubated with 5 nM of mono- or bispecific antibody constructs in flow buffer (DPBS w/o Ca and Mg (PAN-Biotech) with 3% heat inactivated FBS (Gibco)) for 1 h at 4 C in a V-Bottom 384 well plate (Greiner)