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Clean both vial stoppers with alcohol swabs

The copper content of GHK-Cu formulations has been a theoretical safety consideration, given that excessive copper can be toxic - however, the amounts used in cosmetic and research applications are extremely small, the copper is tightly chelated by the peptide (reducing free copper availability), and topical absorption of copper from these products appears minimal and well below levels that would cause systemic copper accumulation or toxicity

This broad spectrum of activity makes GHK-Cu a molecule of significant interest in preclinical studies related to dermatology, regenerative medicine, and cellular senescence

Use is restricted to qualified research professionals in certified laboratory settings

If you have known allergies or sensitivities, inform your healthcare provider before starting any treatment

NIH/3T3 cells (5 10 cells per well) were seeded onto the hydrogel surface and incubated for 24 h