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Complex formation and purification For the tirzepatideGIPRG s complex, cell pellets were lysed in a buffer containing 20 mM HEPES, 100 mM NaCl, pH 7.4, 10 mM MgCl 2 , 1 mM MnCl 2 and 10% glycerol supplemented with protease inhibitor cocktail, EDTA-free (TragetMol)
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Biochim Biophys Acta 1842:25102516
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